| Rank | Bib | Name | Affiliation | Time | Gap |
| 1 | 21 | VALENTE Jennifer | Twenty20 pb Sho-Air | 1:03:06. | 1:03:06 |
| 2 | 34 | RYAN Kendall | TIBCO-Silicon Valley Bank | 1:03:06. | s.t. |
| 3 | 4 | * WHITE Emma | Rally Cycling | 1:03:06. | s.t. |
| 4 | 13 | PE?UELA Diana | UnitedHealthcare Pro Cycling Te | 1:03:06. | s.t. |
| 5 | 42 | OWEN Harriet | Hagens Berman / Supermint | 1:03:06. | s.t. |
| 6 | 14 | HALL Lauren | UnitedHealthcare Pro Cycling Te | 1:03:06. | s.t. |
| 7 | 3 | BEVERIDGE Allison | Rally Cycling | 1:03:08. | s.t. |
| 8 | 93 | * CLONAN Kristina | Fearless Femme | 1:03:08. | s.t. |
| 9 | 154 | LANGDON Rachel | Team Affinity | 1:03:08. | s.t. |
| 10 | 151 | KILBURG Mia | Team Affinity | 1:03:08. | s.t. |
| 11 | 122 | * MEISELS Esther | Point S Auto p/b Nokian Tyres | 1:03:08. | s.t. |
| 12 | 94 | OLIVIERA Flavia | Fearless Femme | 1:03:08. | s.t. |
| 13 | 92 | ANKUDINOFF Ashlee | Fearless Femme | 1:03:08. | s.t. |
| 14 | 134 | * FRANCO Natalia | Stages Cycling Team | 1:03:09. | s.t. |
| 15 | 6 | MICKEY Abigail | Rally Cycling | 1:03:09. | s.t. |
| 16 | 72 | CORDOVA Lisa | Amy D. Foundation | 1:03:09. | s.t. |
| 17 | 123 | WONG Melanie | Point S Auto p/b Nokian Tyres | 1:03:09. | s.t. |
| 18 | 35 | ALBRECHT Lex | TIBCO-Silicon Valley Bank | 1:03:09. | s.t. |
| 19 | 12 | THOMAS Leah | UnitedHealthcare Pro Cycling Te | 1:03:09. | s.t. |
| 20 | 53 | DOEBEL-HICKOK Krista | Cylance Pro Cycling | 1:03:09. | s.t. |
| 21 | 11 | HALL Katherine | UnitedHealthcare Pro Cycling Te | 1:03:10. | s.t. |
| 22 | 124 | MUEGGE Hanna | Point S Auto p/b Nokian Tyres | 1:03:10. | s.t. |
| 23 | 95 | COMPTON Katherine | Fearless Femme | 1:03:10. | s.t. |
| 24 | 41 | CERRA Jessica | Hagens Berman / Supermint | 1:03:10. | s.t. |
| 25 | 5 | * JASTRAB Megan | Rally Cycling | 1:03:10. | s.t. |
| 26 | 15 | * COLE Janelle | UnitedHealthcare Pro Cycling Te | 1:03:10. | s.t. |
| 27 | 1 | * POIDEVIN Sara | Rally Cycling | 1:03:10. | s.t. |
| 28 | 65 | PLESSING Rachel | ALP Cycles Racing | 1:03:10. | s.t. |
| 29 | 111 | BEARDEN Jillian | Palmares | 1:03:10. | s.t. |
| 30 | 2 | * ELLSAY Gillian | Rally Cycling | 1:03:10. | s.t. |
| 31 | 74 | GILL Haley Louise | Amy D. Foundation | 1:03:11. | s.t. |
| 32 | 23 | POWLESS Shayna | Twenty20 pb Sho-Air | 1:03:11. | s.t. |
| 33 | 76 | WARD Maddy | Amy D. Foundation | 1:03:12. | s.t. |
| 34 | 71 | * VELEZ Eleanor | Amy D. Foundation | 1:03:12. | s.t. |
| 35 | 96 | DONLEY Anne | Fearless Femme | 1:03:12. | s.t. |
| 36 | 106 | MILLARD Alexandra (Lexie) | Orion Racing | 1:03:13. | s.t. |
| 37 | 31 | NEWSOM Emily | TIBCO-Silicon Valley Bank | 1:03:13. | s.t. |
| 38 | 121 | ORTON Beth Ann | Point S Auto p/b Nokian Tyres | 1:03:13. | s.t. |
| 39 | 141 | LUJAN Emma | Swisse Femme ?quipe | 1:03:13. | s.t. |
| 40 | 152 | GANZAR Leigh Ann | Team Affinity | 1:03:13. | s.t. |
| 41 | 113 | * BACKUS Charlotte | Palmares | 1:03:14. | s.t. |
| 42 | 24 | ROORDA Stephanie | Twenty20 pb Sho-Air | 1:03:14. | s.t. |
| 43 | 25 | DRAGOO Allie | Twenty20 pb Sho-Air | 1:03:14. | s.t. |
| 44 | 62 | KARAGIANIS Daphne | ALP Cycles Racing | 1:03:15. | s.t. |
| 45 | 133 | INGLIS Gwen | Stages Cycling Team | 1:03:15. | s.t. |
| 46 | 22 | CLEVENGER Erica | Twenty20 pb Sho-Air | 1:03:15. | s.t. |
| 47 | 44 | BACA Allison | Hagens Berman / Supermint | 1:03:18. | 12 |
| 48 | 101 | JACKSON Carlyn | Orion Racing | 1:04:10. | 1:04 |
| 49 | 115 | LYTLE Hayley | Palmares | 1:04:10. | s.t. |
| 50 | 112 | MANI Caroline | Palmares | 1:04:10. | s.t. |
| 51 | 103 | HALE Jennifer Regan | Orion Racing | 1:04:10. | s.t. |
| 52 | 64 | * ESCOBAR Manuela | ALP Cycles Racing | 1:04:10. | s.t. |
| 53 | 32 | TETRICK Jennifer | TIBCO-Silicon Valley Bank | 1:04:10. | s.t. |
| 54 | 33 | BUSS Kathryn | TIBCO-Silicon Valley Bank | 1:04:11. | s.t. |
| 55 | 61 | ANDERSON Lynne | ALP Cycles Racing | 1:04:11. | s.t. |
| 56 | 75 | * SOH Jasmine | Amy D. Foundation | 1:04:11. | s.t. |
| 57 | 52 | BRECK Holly | Cylance Pro Cycling | 1:04:11. | s.t. |
| 58 | 136 | KUHAJEK Jeannie | Stages Cycling Team | 1:04:11. | s.t. |
| 59 | 66 | KVROUWENVELDER Kristina | ALP Cycles Racing | 1:04:11. | s.t. |
| 60 | 143 | * MARCOLINI Emily | Swisse Femme ?quipe | 1:04:47. | 1:41 |
| 61 | 91 | WIASAK Rebecca | Fearless Femme | 1:05:14. | 2:08 |
| 62 | 73 | * DINAN Clio | Amy D. Foundation | 1:05:16. | s.t. |
| 63 | 63 | THOMAS Andrea | ALP Cycles Racing | 1:05:51. | 2:45 |
| 64 | 102 | SEIPP Carol Jeane | Orion Racing | 1:07:31. | 4:25 |
| 65 | 146 | * PAYER Kira | Swisse Femme ?quipe | 1:08:22. | 5:16 |
| 66 | 104 | RUSSELL Gabrielle | Orion Racing | 1:10:07. | 7:01 |
| DNS | 43 | LUEBKE Jen | Hagens Berman / Supermint | | |
